pnt1a human prostate epithelial cells  (Millipore)

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: The secretion of testosterone ( a , b ), estradiol ( c , d ), and progesterone ( e , f ) after 48 h of exposure to AOH and DHEA, evaluated by ELISA tests in PC3 and PNT1A cells. Results are presented as mean ± SE. One-way ANOVA was used to perform statistical analysis of the results, and p < 0.05 was considered statistically significant. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: Effects of AOH, DHEA, and co-treatment of AOH and DHEA on the protein expression of CAV-1. The results are presented as fold changes in the expression. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, CAV-1—caveolin 1.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: AOH, DHEA, as well as co-treatment induce apoptosis after 48 h of exposure in PC3 and PNT1A cells ( a ). Analysis of apoptotic cells based on flow cytometry with representative results ( b ). One-way ANOVA was used for statistical analysis. Results are presented and mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001, as compared to the control. Representative results of Western blot analysis of CASP3 ( c ). Analysis of the relative expression of ANX5a ( d ) and TP53 genes in normal and prostate cancer cell lines ( e ). AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, Casp3—caspase 3.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Flow Cytometry, Control, Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: Effects of AOH, DHEA, and co-treatment of AOH and DHEA on the protein expression of Casp3 and Cleaved Casp3. The results are presented as fold changes in the expression. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control, Casp3—caspase 3.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Revealing the Role of Alternariol in the Local Steroidogenesis in Human Prostate Normal and Cancer Cells

doi: 10.3390/ijms24119513

Figure Lengend Snippet: AOH, DHEA, as well as co-treatment induce changes in cell cycle progression after 48 h of exposure in PNT1A ( a ) and PC3 cells ( b ). Analysis based on the results obtained in flow cytometry. Analysis of the relative expression of CDKN1A ( c ) and CDK2 genes in normal and prostate cancer cell lines ( d ). One-way ANOVA was used for statistical analysis. The results are presented as mean ± SE. * p < 0.05, ** p < 0.01, *** p < 0.001. AOH—alternariol, DHEA—dehydroepiandrosterone, Cnt—control. The results were acquired in three independent experiments.

Article Snippet: The normal human prostate epithelial cell line PNT1A and the prostate adenocarcinoma cell line PC3 were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Sigma-Aldrich, Saint Louis, MO, USA) and maintained in a humidified incubator (37 °C, 5% CO 2 ).

Techniques: Flow Cytometry, Expressing, Control

Journal: The Journal of Biological Chemistry

Article Title: Leucine-rich repeat–containing G protein–coupled receptor 4 (Lgr4) is necessary for prostate cancer metastasis via epithelial–mesenchymal transition

doi: 10.1074/jbc.M116.771931

Figure Lengend Snippet: LGR4 is overexpressed in human prostate cancer and positively correlated with cancer recurrence. A and B, LGR4 expression was assessed by qPCR (A) and Western blotting (n = 3) (B) in different human prostate cancer cell lines. C, the LGR4 mRNA level was analyzed using GEO accession no. GSE6099 (33). p = 0.007. D, the Kaplan–Meier disease-free survival curve was generated using GEO accession no. GSE3933 (34). LGR4low, n = 28; LGR4high, n = 30. E, the Kaplan–Meier disease-free survival curve was generated by using GEO accession no. GSE10645 (35). p < 10E−6; LGR4low, n = 59; LGR4high, n = 60. The data in A are expressed as means ± S.E. of three independent experiments (*, p < 0.05; **, p < 0.01; versus PNT1A control).

Article Snippet: Human prostate cancer LNCAP, PC3, DU145, MDA-PCa-2a, MDA-PCa-2b, LAPC-4, and VCAP cells and PNT1A human normal prostate epithelial cells were purchased from the ATCC and cultured as described previously ( 56 ) without contamination such as mycoplasma.

Techniques: Expressing, Western Blot, Generated, Control